Pramanicin induces apoptosis in Jurkat leukemia cells; a role for JNK, p38 and caspase activation

Pramanicin is a novel anti-fungal drug with a wide range of potential application against human diseases. It has been previously shown that pramanicin induces cell death and increases calcium levels in vascular endothelial cells. In the present study, we showed that pramanicin induced apoptosis in Jurkat T leukemia cells in a dose- and time-dependent manner. Our data reveal that pramanicin induced the release of cytochrome c and caspase-9 and caspase-3 activation, as evidenced by detection of active caspase fragments and fluorometric caspase assays. Pramanicin also activated c-jun N-terminal kinase (JNK), p38 and extracellular signal-regulated kinases (ERK 1/2) with different time and dose kinetics. Treatment of cells with specific MAP kinase and caspase inhibitors further confirmed the mechanistic involvement of these signalling cascades in pramanicin-induced apoptosis. JNK and p38 pathways acted as pro-apoptotic signalling pathways in pramanicin-induced apoptosis, in which they regulated release of cytochrome c and caspase activation. In contrast the ERK 1/2 pathway exerted a protective effect through inhibition of cytochrome c leakage from mitochondria and caspase activation, which were only observed when lower concentrations of pramanicin were used as apoptosis-inducing agent and which were masked by the intense apoptosis induction by higher concentrations of pramanicin. These results suggest pramanicin as a potential apoptosis-inducing small molecule, which acts through a well-defined JNK- and p38-dependent apoptosis signalling pathway in Jurkat T leukemia cells

Self-dual solutions of Yang-Mills theory on Euclidean AdS space

We find non-trivial, time-dependent solutions of the (anti) self-dual Yang-Mills equations in the four dimensional Euclidean Anti-de Sitter space. In contrast to the Euclidean flat space, the action depends on the moduli parameters and the charge can take any non-integer value.Comment: 11 pages, 2 figures; version 2: new references added, to appear in PR

The role of nailfold capillaroscopy in interstitial lung diseases - Can it differentiate idiopathic cases from collagen tissue disease associated interstitial lung diseases?

Introduction: Nailfold capillaroscopy (NFC) is a non-invasive diagnostic test that is mostly used for early diagnosis of collagen tissue diseases (CTDs). We aimed to evaluate whether NFC findings could be a clue for discriminating idiopathic interstitial lung diseases (ILD) from CTD associated ILDs (CTD-ILD). Additionally it was aimed to determine whether NFC could be helpful in discriminating usual interstitial pneumonia (UIP) pattern from non-specific interstitial pneumonia (NSIP) pattern. Materials and Methods: We grouped patients into three main groups: 15 CTD-ILD, 18 idiopathic ILD, and 17 patients in the control group. The CTD-ILD group was split into two subgroups: 8 patients with Sjögren’s syndrome (SJS)-associated ILD and 7 with rheumatoid arthritis (RA)-associated ILD. The idiopathic-ILD group consisted of 10 idiopathic NSIP and 8 IPF patients. The control group consisted of 10 SJS and 7 RA patients without lung disease. None of the patients were on acute exacerbation at the time of examination, and none had Reynaud’s phenomenon. Results: Mean capillary density was significantly reduced only in the CTD-ILD group as compared to the control group (p= 0.006). In subgroup analysis, it was determined that RA-ILD, IPF, and SJSILD subgroups had more severe capillaroscopic abnormalities. Mean capillary density in patients with the UIP pattern was reduced compared to patients with the NSIP pattern and those in the control group; p values were 0.008 and < 0.001, respectively. Conclusion: This study is to be the first describing and comparing the nailfold capillaroscopic findings of patients with NSIP and UIP patterns. NFC findings can be helpful in discriminating UIP patterns from NSIP patterns. But to show its role in differentiating idiopathic disease, more studies with more patients are needed. © 2015, Ankara University. All rights reserved

Cylindrically symmetric, static strings with a cosmological constant in Brans-Dicke theory

The static, cylindrically symmetric vacuum solutions with a cosmological constant in the framework of the Brans-Dicke theory are investigated. Some of these solutions admitting Lorentz boost invariance along the symmetry axis correspond to local, straight cosmic strings with a cosmological constant. Some physical properties of such solutions are studied. These strings apply attractive or repulsive forces on the test particles. A smooth matching is also performed with a recently introduced interior thick string solution with a cosmological constant.Comment: 8 pages, Revtex; Published versio

Cylindrically Symmetric Vacuum Solutions in Higher Dimensional Brans-Dicke Theory

Higher dimensional, static, cylindrically symmetric vacuum solutions with and without a cosmological constant in the Brans-Dicke theory are presented. We show that, for a negative cosmological constant and for specific values of the parameters, a particular subclass of these solutions include higher dimensional topological black hole-type solutions with a flat horizon topology. We briefly extend our discussion to stationary vacuum and $\Lambda-$vacuum solutions.Comment: V3: Published Versio

Two-Frequency Jahn-Teller Systems in Circuit QED

We investigate the simulation of Jahn-Teller models with two non-degenerate vibrational modes using a circuit QED architecture. Typical Jahn-Teller systems are anisotropic and require at least a two-frequency description. The proposed simulator consists of two superconducting lumped-element resonators interacting with a common flux qubit in the ultrastrong coupling regime. We translate the circuit QED model of the system to a two-frequency Jahn-Teller Hamiltonian and calculate its energy eigenvalues and the emission spectrum of the cavities. It is shown that the system can be systematically tuned to an effective single mode Hamiltonian from the two-mode model by varying the coupling strength between the resonators. The flexibility in manipulating the parameters of the circuit QED simulator permits isolating the effective single frequency and pure two-frequency effects in the spectral response of Jahn-Teller systems.Comment: 8 pages, 4 figures, figures revise

Factorization of Joint Probability Mass Functions into Parity Check Interactions

We show that any joint probability mass function (PMF) can be expressed as a product of parity check factors and factors of degree one with the help of some auxiliary variables, if the alphabet size is appropriate for defining a parity check equation. In other words, marginalization of a joint PMF is equivalent to a soft decoding task as long as a finite field can be constructed over the alphabet of the PMF. In factor graph terminology this claim means that a factor graph representing such a joint PMF always has an equivalent Tanner graph. We provide a systematic method based on the Hilbert space of PMFs and orthogonal projections for obtaining this factorization.Comment: 5 pages, 1 figures, appeared in the proceedings of ISIT 2009; Changed content, more recent version than as appeared in the proceeding

Godel-type Metrics in Various Dimensions II: Inclusion of a Dilaton Field

This is the continuation of an earlier work where Godel-type metrics were defined and used for producing new solutions in various dimensions. Here a simplifying technical assumption is relaxed which, among other things, basically amounts to introducing a dilaton field to the models considered. It is explicitly shown that the conformally transformed Godel-type metrics can be used in solving a rather general class of Einstein-Maxwell-dilaton-3-form field theories in D >= 6 dimensions. All field equations can be reduced to a simple "Maxwell equation" in the relevant (D-1)-dimensional Riemannian background due to a neat construction that relates the matter fields. These tools are then used in obtaining exact solutions to the bosonic parts of various supergravity theories. It is shown that there is a wide range of suitable backgrounds that can be used in producing solutions. For the specific case of (D-1)-dimensional trivially flat Riemannian backgrounds, the D-dimensional generalizations of the well known Majumdar-Papapetrou metrics of general relativity arise naturally.Comment: REVTeX4, 17 pp., no figures, a few clarifying remarks added and grammatical errors correcte

Lessons from Mission-Critical Spreadsheets

We present eighteen examples of mission-critical spreadsheets used by diverse people and organizations for application software development, financial risk management, executive information systems, sales and marketing business processes, business operations, and complex analytics. We argue the spreadsheet is a Rapid Development Language, an Integrated Development Environment, and a Fourth Generation Language, and has unusual challenges regarding source code protection. We note that intentional spreadsheet applications are largely absent from the error literature. We explain why people might prefer a spreadsheet to an application developed by the IT department, and show how some spreadsheet programmers choose to avoid--or do not have--an IT department. We find that 1) Spreadsheets are widely used for mission-critical functions; 2) Spreadsheets are an effective application development platform; 3) There is diversity of development skill in creators of mission-critical spreadsheets; 4) Sophisticated programmers sometimes choose spreadsheets over other languages; 5) Spreadsheets are amenable to formal development practices, but such practices seem rare; 6) Spreadsheets play a central role in the evolution of business processes and work systems; and 7) Spreadsheets are a source of accidental legacy systems . We provide the Skill-User Programming Paradigm to help interpret and explain our observations. We conclude that spreadsheets are vitally important to business, and merit sustained research to discover techniques to enhance quality, productivity, and maintainability